The fact that Primo vessel by a new circulatory system exists in the lymphatic vessel is confirmed the anatomy of rabbit. The isolated Primo vessel is investigated by the morphological features.

Before the anatomy, rabbit inside of cage box lay on the sound wave vibrating apparatus is applied by the various frequency and acceleration during 30 min . The isolation and observation of Primo vessel by the activity of lymphatic circulatory system affected at a special frequency and acceleration of 7 Hz and 1.07 m/s² is better than one with the sonic wave vibration. The probability of observation for Primo vessel enhanced to 90 %.

Therefore, the reproducible isolation and observation method of Primo vessel needs the major process such as sonic Wave therapy by the sonic wave vibration apparatus.